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Rubber policeman sigma.
Hydrochloric acid hcl sodium hydroxide naoh ethylenediaminetetraacetic acid edta dithiothreitol dtt sodium chloride.
Adherent cells ks y 1 m21 hey t1 were harvested on the following day using a rubber policeman.
Phosphate buffered saline ph 7 2 with mg ca pbs sigma magnesium chloride mgcl 2 calcium chloride cacl 2 sodium dodecyl sulfate sds tris base.
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The cells were scraped with a sterile rubber policeman sigma aldrich collected in pbs in 1 5 ml tubes eppendorf and stored at 80 c.
The common type of it is attached to a glass rod and used in chemical laboratories to transfer residues of precipitate or solid on glass surfaces when performing gravimetric analysis this equipment works well under gentle delicate and precise requirement.
A policeman also comes in various sizes shapes and types.
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Find null z168661 msds related peer reviewed papers technical documents similar products more at sigma aldrich.
The instruments feature a flat.
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Viable cell counts were determined by trypan blue dye exclusion.
The versatile double ended styles have two different shaped blades for precise tailoring to specific testing applications.
Ideal for scraping and mixing transfer collect or stir biological samples with complete ease by using the multi purpose spatulas.
Qiashredder spin columns qiagen 3.
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Cells grown in suspension u937 hl 60 a6876 p3hr1 and adherent cells were transferred to 12 15 mm round bottomed centrifuge tubes.
After 24 hrs cells were washed 3 times with ice cold tris buffered saline tbs collected in tbs using a rubber policeman and pelleted by centrifugation 1500 rpm.
A policeman is a hand held flexible natural rubber or plastic scraper.
Blunt tapered or round spatula tips in any size are designed to not scratch or damage surface when handling.
Each cell pellet was lysed in 112 5 μl of lysis buffer 10 mm tris ph 8 0 10 mm edta ph 8 0 1 np 40 1 mm pmsf for 20 min at 4 c diluted with 634 5 μl of dilution buffer 10 mm tris ph 8 0 and.
Natural rubber scrapers rod diam.
